Vitrification of Pronuclear Zygotes Perturbs Porcine Zygotic Genome Activation

Animals

Jun Jiao¹  Zubing Cao²  Yelian Yan²  Xin Wang²  Qiuchen Liu²  Chengxue Liu²  Tong Yu²  Tengteng Xu²  Yangyang Ma²  Yunhai Zhang²  Mengya Zhang²  Anucha Sathanawongs³ 

[1] Anhui Haoyu Animal Husbandry Co., Ltd., Luan 237451, China;Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand;

关键词: pig;    vitrification;    embryo;    zygotic genome activation;    blastocyst;   

DOI  :  10.3390/ani12050610

来源: DOAJ

【 摘 要 】

Zygotic genome activation (ZGA) plays an essential role in early embryonic development. Vitrification is a common assisted reproductive technology that frequently reduces the developmental competence of embryos. However, the effect of vitrification on porcine ZGA and gene expression during ZGA remains largely unclear. Here, we found that vitrification of pronuclear zygotes derived from parthenogenetic activation (PA) and in vitro fertilization (IVF) resulted in a significant reduction in the rates of 2-cell, 4-cell, and blastocysts, but did not affect the quality of blastocysts. Functional research revealed that RNA polymerase II Inhibitor (α-amanitin) treatment significantly reduced global transcriptional activity and developmental efficiency of both 4-cell and 8-cell embryos, implying an essential role of ZGA in porcine early embryonic development. Furthermore, vitrification did not affect the synthesis of nascent mRNA of 2-cell embryos, but significantly inhibited global transcriptional activity of both 4-cell and 8-cell embryos, suggesting an impaired effect of vitrification on porcine ZGA. Correspondingly, the single-cell analysis showed that vitrification caused the downregulation or upregulation expression of maternal genes in 4-cell embryos, also significantly decreased the expression of zygotic genes. Taken together, these results indicated that vitrification of pronuclear zygotes impairs porcine zygotic genome activation.

【 授权许可】

Unknown