Viability after vitrification of sow and sheep embryos produced in vitro

Revista de Salud Animal

Rodríguez Suastégui, J.L¹  Hernández Pichardo, J.E¹  Fernández Reyes, F¹  Romero Ramírez, J.G¹ 

[1] Universidad Autónoma Metropolitana-Xochimilco (UAM-X), D.F., México

关键词: sow;    sheep;    vitrification;    viability;    embryo;    in vitro maturation;    in vitro fertilization;   

DOI  :  

学科分类：兽医学

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

This study was aimed to assess the viability after vitrification of sow and sheep embryos produced in vitro. Complexes Oocyte-cumulus cells (COCs) were obtained from ovaries collected from sows and sheeps in the slaughterhouse. The viability was evaluated in three groups of embryos: unvitrified (control group), after vitrification (warming) and at 72 hours of culture after warming. The vitrification used two steps with ethylene glycol (EG) 4% (v/v), fetal bovine serum (FBS) 20%, in TCM-199 at 37 °C for 15 minutes, then the embryos were transferred to a vitrification solution of 35% EG, 0.4 M trehalose, and 20% FBS in TCM-199 for 20 seconds and finally placed in beveled edge open straws (BES). The straws were immersed and kept in liquid nitrogen for a week. The total viability of the vitrified/warmed embryos from sows was 49.3%, and the highest viabilities were shown by the 8-16 blastomere embryos (63.7%) and the morulae (61,7%), though the differences were not statistically significant. The total viability in the control group was of 74.3%, and the 8-16 blastomere embryos and morulae had 100% of viability. The total viability of the vitrified/warmed embryos from sheeps was 63.1%, and the highest viabilities were shown by the morulae (76.1%) and the 8-16 blastomere embryos (68.1%), though the differences were not statistically significant. The total viability in the control group was 100% in all the different stages of the embryonic development. The viability obtained after culturing the vitrified/warmed embryos for 72 hours was 1.5% of sow morulae and 2.8% of sheep blastocysts; in the latter, two morulae changed to blastocysts. It is concluded that the vitrification procedure in beveled edge open straws using ethylene glycol and trehalose as cryoprotectants allowed recovering of sow and sheep embryos viable at the devitrifying moment, but their further development is reduced.

【 授权许可】

Unknown