MATURATION AND FERTILIZATION IN VITRO OF SOW OOCYTES OBTAINED FOR CUT FOLLICLES AND PUNCTURE

Revista de Salud Animal

Reyes Flores, María del Carmen²  Hernández Pichardo, J. E¹  Fernández Reyes, F¹ 

[1] Universidad Autónoma Metropolitana-Xochimilco (UAM-X), México;Clínica Privada

关键词: sow;    cocyte;    embryo;    maturation in vitro;    fertilization in vitro     INTRODUCCIÓNLos ovarios de los mamíferos contienen folículos que se encuentran en diferentes estadios de desarrollo;    de los cuáles solamente una pequeña proporción va a ser utilizada durante la vida reproductiva del animal (1) La recolección de ovocitos permite recuperar y aprovechar folículos no ovulatorios;    que bajo condiciones fisiológicas se tornarían en folículos atrésicos;    con el fin de aprovechar el máximo potencial genético de una donadora por procedimiento in vitro (2);   

DOI  :  

学科分类：兽医学

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

A number of 1331 oocytes were recovered from ovaries obtained in slaughterhouses; from these 670 were obtained by puncture and 661 by follicles cutting, then cultivated in the medium defined suplemented TCM-199. Maturation was evaluated by fixation and staining with acetic orceín from 302 ovocytes fixed by puncture method 97 matured and from 294 oocytes fixed by cutting method 95 matured corresponding 32,1 and 32 from maturation percentage respectively, between both groups. A number 368 oocytes from the puncture method and 367 from the cutting one were fertilized in , TBM buffered medium with Tris and then transfered to the development embryo medium NCSU-23. The obtained results were 40 embryos in those of method of puncture and 36 embryos in cutting method, corresponding 11,8% and 11,2% of embryonic development respectively, not observing any significant statistical difference (P>0.05), between both groups. One concludes that the efficiency in both procedures of obtaining of oocytes for maturation in vitro of oocytes of pig and its fertilization in vitro is equal, since they significantly did not affect the percentage in the maturation and embryonic development in vitro.

【 授权许可】

CC BY-NC