Biological Effects of C60 Fullerene Revealed with Bacterial Biosensor—Toxic or Rather Antioxidant?

Biosensors

Sergey Emelyantsev¹  Evgeniya Prazdnova¹  Igor Alperovich¹  Vladimir Chistyakov¹ 

[1] Academy of Biology and Biotechnologies, Southern Federal University, 344090 Rostov-on-Don, Russia;

关键词: fullerene C₆₀;    antioxidant activity;    toxicity;    oxidative stress;    reactive oxygen species;    hydrogen peroxide;    Escherichia coli;    biosensors;    bioluminescence assay;   

DOI  :  10.3390/bios9020081

来源: DOAJ

【 摘 要 】

Nanoparticles have been attracting growing interest for both their antioxidant and toxic effects. Their exact action on cells strongly depends on many factors, including experimental conditions, preparation, and solvents used, which have contributed to the confusion regarding their safety and possible health benefits. In order to clarify the biological effects of the most abundant fullerene C₆₀, its impact on the Escherichia coli model has been studied. The main question was if C₆₀ would have any antioxidant influence on the cell and, if yes, whether and to which extent it would be concentration-dependent. An oxidative stress induced by adding hydrogen peroxide was measured with an E. coli MG1655 pKatG-lux strain sensor, with its time evolution being recorded in the presence of fullerene C₆₀ suspensions of different concentrations. Optimal conditions for the fullerene C₆₀ solubilization in TWEEN 80 2% aqueous solution, together with resulting aggregate sizes, were determined. Results obtained for the bacterial model can be extrapolated on eukaryote mitochondria. The ability of C₆₀ to penetrate through biological membranes, conduct protons, and interact with free radicals is likely responsible for its protective effect detected for E. coli. Thus, fullerene can be considered as a mitochondria-targeted antioxidant, worth further researching as a prospective component of novel medications.

【 授权许可】

Unknown