Toxicity and Antioxidant Activity of Fullerenol C60,70 with Low Number of Oxygen Substituents

International Journal of Molecular Sciences

卷:22

Arina G. Kicheeva¹  Nadezhda S. Kudryasheva²  Ekaterina S. Kovel²  Evsei A. Stepin³  Grigory N. Churilov⁴  Natalia G. Vnukova⁴ 

[1] FRC KSC SB RAS, 660036 Krasnoyarsk, Russia;

[2] Institute of Biophysics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, Russia;

[3] Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia;

[4] Institute of Physics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, Russia;

关键词: fullerenol;    toxicity;    antioxidant activity;    reactive oxygen species;    bioluminescent assay;    hormesis;   

DOI  :  10.3390/ijms22126382

来源: DOAJ

【 摘 要 】

Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene’s carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system.

【 授权许可】

Unknown