HIF-1α promotes the migration and invasion of hepatocellular carcinoma cells via the IL-8–NF-κB axis

Cellular & Molecular Biology Letters

Huihui Guo¹  Tao Xue¹  Hui Gong¹  Sanxiong Huang²  Chengwu Tang³  Wenming Feng³  Qilin Shi⁴  Ge Cui⁴  Guanghui Yang⁵ 

[1] Central Laboratory, The First People’s Hospital of Huzhou;Department of General Surgery, The First People’s Hospital of Huzhou;Department of Hepatobiliary Pancreatic Surgery, The First People’s Hospital of Huzhou;Department of Pathology, The First People’s Hospital of Huzhou;Department of Pharmacy, The First People’s Hospital of Huzhou;

关键词: Hepatocellular carcinoma;    Hypoxia;    HIF-1α;    IL-8;    NF-κB;   

DOI  :  10.1186/s11658-018-0077-1

来源: DOAJ

【 摘 要 】

Abstract Background Hypoxia plays a critical role in many cancers. Hypoxia inducible factor-1α (HIF-1α) is an important mediator of the hypoxia response. It regulates the expression of various chemokines involved in tumor growth, angiogenesis and metastasis but the associated pathway needs further investigation. Methods The expression level of HIF-1α was determined in hepatocellular carcinoma (HCC) cells. The correlation of interleukin-8 (IL-8) and HIF-1α was assessed by knocking down HIF-1α. These cells were also used to assess its influence on HCC cell migration and invasion was checked. Pyrrolidinedithiocarbamate (PDTC), an inhibitor of NF-κB, was used to confirm the associated signaling pathway. Results HIF-1α was significantly expressed in HCC cells and found to promote HCC cell migration and invasion in an IL-8-dependent manner. NF-κB was confirmed to be involved in the process. Conclusions HIF-1α promotes HCC cell migration and invasion by modulating IL-8 via the NF-κB pathway.

【 授权许可】

Unknown