Metabolite Profiling of Aquilaria malaccensis Leaf Extract Using Liquid Chromatography-Q-TOF-Mass Spectrometry and Investigation of Its Potential Antilipoxygenase Activity In-Vitro

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NorMalia Abd Warif¹  MuhammadLokman Md. Isa²  SaripahS. S. Abd-Azziz³  HamzahMohd. Salleh⁴  ManarA. Eissa⁴  YumiZ. H-Y. Hashim⁴  DinaM. El-Kersh⁵ 

[1] Biomedical Sciences Program, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia;Department of Basic Medical Sciences for Nursing, Kulliyah of Nursing, International Islamic University Malaysia, Kuantan 25200, Pahang, Malaysia;Faculty of Science and Mathematics, Sultan Idris Education University, Tanjung Malim 35900, Perak, Malaysia;International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, Gombak 53100, Selangor, Malaysia;Pharmacognosy Department, Faculty of Pharmacy, The British University in Egypt, Cairo 11837, Egypt;

关键词: aquilaria malaccensis;    agarwood;    extract;    lcms;    gcms;    anti-inflammatory;    toxicity;    oec;    wst-1;   

DOI  :  10.3390/pr8020202

来源: DOAJ

【 摘 要 】

The Aquilaria malaccensis species of the genus Aquilaria is an abundant source of agarwood resin and many bioactive phytochemicals. Recent data regarding the chemical constituents and biological activities of Aquilaria leaves led us to attempt to qualitatively profile the metabolites of Aquilaria malaccensis leaves from a healthy, noninoculated tree through phytochemical screening, GC-MS, and LC/Q-TOF-MS. The present work is also the first to report the antilipoxygenase activity of A. malaccensis leaves from healthy noninoculated tree and investigate its toxicity on oral mucosal cells. A total of 53 compounds were tentatively identified in the extract, some of which have been described in literature as exhibiting anti-inflammatory activity. A number of compounds were identified for the first time in the extract of A. malaccensis leaf, including quercetin, quercetin-O-hexoside, kaempferol-O-dirhamnoside, isorhamnetin-O-hexoside, syringetin-O-hexoside, myricetin, tetrahydroxyflavanone, hesperetin, sissotrin, and lupeol. The antilipoxygenase assay was used to determine the lipoxygenase (LOX) inhibitory potential of the extract, while a WST-1 assay was conducted to investigate the effect of the extract on oral epithelial cells (OEC). The extract implied moderate anti-LOX activity with IC₅₀ value of 71.6 µg/mL. Meanwhile, the cell viability of OEC ranged between 92.55% (10 µg/mL)−76.06% ± (100 µg/mL) upon treatment, indicating some potential toxicity risks. The results attained encourage future studies of the isolation of bioactive compounds from Aquilaria malaccensis leaves, as well as further investigation on the anti-inflammatory mechanisms and toxicity associated with their use.

【 授权许可】

Unknown