Syntrophic Hydrocarbon Degradation in a Decommissioned Off-Shore Subsea Oil Storage Structure

Microorganisms

Perrine Cruaud¹  IanM. Head²  Nicolas Tsesmetzis³  Adrien Vigneron³  Frederic Ducellier⁴ 

[1] Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC G1S 0A6, Canada;School of Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK;Shell International Exploration and Production Inc., Houston, TX 77082, USA;Société des Pétroles Shell, 92708 Colombes, France;

关键词: MAGs;    metagenomics;    oil systems;    bioremediation;    marine environments;    alkanes;   

DOI  :  10.3390/microorganisms9020356

来源: DOAJ

【 摘 要 】

Over the last decade, metagenomic studies have revealed the impact of oil production on the microbial ecology of petroleum reservoirs. However, despite their fundamental roles in bioremediation of hydrocarbons, biocorrosion, biofouling and hydrogen sulfide production, oil field and oil production infrastructure microbiomes are poorly explored. Understanding of microbial activities within oil production facilities is therefore crucial for environmental risk mitigation, most notably during decommissioning. The analysis of the planktonic microbial community from the aqueous phase of a subsea oil-storage structure was conducted. This concrete structure was part of the production platform of the Brent oil field (North Sea), which is currently undergoing decommissioning. Quantification and sequencing of microbial 16S rRNA genes, metagenomic analysis and reconstruction of metagenome assembled genomes (MAGs) revealed a unique microbiome, strongly dominated by organisms related to Dethiosulfatibacter and Cloacimonadetes. Consistent with the hydrocarbon content in the aqueous phase of the structure, a strong potential for degradation of low molecular weight aromatic hydrocarbons was apparent in the microbial community. These degradation pathways were associated with taxonomically diverse microorganisms, including the predominant Dethiosulfatibacter and Cloacimonadetes lineages, expanding the list of potential hydrocarbon degraders. Genes associated with direct and indirect interspecies exchanges (multiheme type-C cytochromes, hydrogenases and formate/acetate metabolism) were widespread in the community, suggesting potential syntrophic hydrocarbon degradation processes in the system. Our results illustrate the importance of genomic data for informing decommissioning strategies in marine environments and reveal that hydrocarbon-degrading community composition and metabolisms in man-made marine structures might differ markedly from natural hydrocarbon-rich marine environments.

【 授权许可】

Unknown