Comparative metagenomics at Solfatara and Pisciarelli hydrothermal systems in Italy reveal that ecological differences across substrates are not ubiquitous

Frontiers in Microbiology

Microbiology

Mihaela Glamoclija¹  Ifeoma R. Ugwuanyi¹  Marilyn L. Fogel²  Andrew Steele³  Roxane Bowden³  Monica Piochi⁴  Angela Mormone⁴  Giuseppe De Natale⁵  Claudia Troise⁵  Renato Somma⁶ 

[1] Department of Earth and Environmental Sciences, Rutgers University, Newark, NJ, United States;EDGE Institute, University of California, Riverside, Riverside, CA, United States;Earth and Planets Laboratory, Carnegie Institution for Science, Washington, DC, United States;Istituto Nazionale di Geofisica e Vulcanologia, Osservatorio Vesuviano, Naples, Italy;Istituto Nazionale di Geofisica e Vulcanologia, Osservatorio Vesuviano, Naples, Italy;Consiglio Nazionale delle Ricerche INO, Naples, Italy;Istituto Nazionale di Geofisica e Vulcanologia, Osservatorio Vesuviano, Naples, Italy;Consiglio Nazionale delle Ricerche IRISS, Naples, Italy;

关键词: hydrothermal system;    Solfatara;    Pisciarelli;    microbial diversity;    metagenomics;    MAGs;   

DOI  :  10.3389/fmicb.2023.1066406

 received in 2022-10-10, accepted in 2023-01-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionContinental hydrothermal systems (CHSs) are geochemically complex, and they support microbial communities that vary across substrates. However, our understanding of these variations across the complete range of substrates in CHS is limited because many previous studies have focused predominantly on aqueous settings.MethodsHere we used metagenomes in the context of their environmental geochemistry to investigate the ecology of different substrates (i.e., water, mud and fumarolic deposits) from Solfatara and Pisciarelli.Results and DiscussionResults indicate that both locations are lithologically similar with distinct fluid geochemistry. In particular, all substrates from Solfatara have similar chemistry whereas Pisciarelli substrates have varying chemistry; with water and mud from bubbling pools exhibiting high SO42− and NH4+ concentrations. Species alpha diversity was found to be different between locations but not across substrates, and pH was shown to be the most important driver of both diversity and microbial community composition. Based on cluster analysis, microbial community structure differed significantly between Pisciarelli substrates but not between Solfatara substrates. Pisciarelli mud pools, were dominated by (hyper)thermophilic archaea, and on average, bacteria dominated Pisciarelli fumarolic deposits and all investigated Solfatara environments. Carbon fixation and sulfur oxidation were the most important metabolic pathways fueled by volcanic outgassing at both locations. Together, results demonstrate that ecological differences across substrates are not a widespread phenomenon but specific to the system. Therefore, this study demonstrates the importance of analyzing different substrates of a CHS to understand the full range of microbial ecology to avoid biased ecological assessments.

【 授权许可】

Unknown   
Copyright © 2023 Ugwuanyi, Fogel, Bowden, Steele, De Natale, Troise, Somma, Piochi, Mormone and Glamoclija.