Expression of heat-resistant β-glucosidase in Escherichia coli and its application in the production of gardenia blue

Synthetic and Systems Biotechnology

Wenxi Li¹  Yan Huang¹  Xinyu Liu¹  Xinyu Li¹  Shuqi Xu¹  Jielin Li¹  Zirui Ou¹  Zening Xiao¹  Ying Xu²  Jiaqi Huang³  Xiaoli Long⁴  Weizhao Chen⁵ 

[1] College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China;College of Materials Science and Engineering, Shenzhen University, Shenzhen, 518060, China;College of Mechatronics and Control Engineering, Shenzhen University, Shenzhen, 518060, China;Health Science Center School of Biomedical Engineering, Shenzhen University, Shenzhen, 518060, China;Shenzhen Key Laboratory for Microbial Gene Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China;

关键词: β-glucosidase;    Gardenia blue;    Geniposide;    Orthogonal experiment;   

DOI  :  

来源: DOAJ

【 摘 要 】

Gardenia blue is a natural blue pigment that is environmentally friendly, non-toxic, and stable. The hydrolysis of geniposide, catalyzed by β-glucosidase, is a critical step in the production process of gardenia blue. However, β-glucosidase is not resistant to high temperatures, limiting the production of gardenia blue. In this study, we investigated the effectiveness of a heat-resistant glucosidase obtained from Thermotoga maritima in the production of gardenia blue. The enzyme exhibited a maximum activity of 10.60 U/mL at 90 °C. Single-factor and orthogonal analyses showed that exogenously expressed heat-resistant glucosidase reacted with 470.3 μg/mL geniposide and 13.5 μg/mL glycine at 94.2 °C, producing a maximum yield of 26.2857 μg/mL of gardenia blue after 156.6 min. When applied to the dyeing of denim, gardenia blue produced by this method yielded excellent results; the best color-fastness was achieved when an iron ion mordant was used. This study revealed the feasibility and application potential of microbial production of gardenia blue.

【 授权许可】

Unknown