Practices of Translational Medicine in Chinese Herbal Compound: Study on Pharmacological Material Basis of Qingganning Tablet based on HPLC

Journal of International Translational Medicine

Yu-ping XIONG¹  Ya-jie TIAN¹  Ming-zhi WANG²  Chao ZHANG²  Shui-sheng ZHONG² 

[1] School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, 450008, China;Suzhou Institute for Food and Drug Control, Suzhou, Jiangsu, 215104, China;

关键词: Translational medicine;    Chinese medicine;    Qingganning Tablet;    Puerarin;    Geniposide;    Paeoniflorin;    HPLC;   

DOI  :  10.11910/2227-6394.2015.03.04.09

来源: DOAJ

【 摘 要 】

Abstract Objective: To provide a stable and reliable analysis method for the study on pharmacological material basis of Qingganning Tablet (Tablet for Clearing Away Liver Heat). Methods: High-performance liquid chromatography (HPLC) was used to determine the content of puerarin, geniposide and paeoniflorin in Qingganning Tablet, Hypersil BDS C18 (250 mm×4.6 mm, 5 μm); mobile phase: acetonitrile-water (16:84); flow velocity: 1.0 mL·min^-1; detection wavelength: 230 nm; and column temperature: 35℃. with detection conditions being: chromatographic column: Results: The detection concentration of puerarin, geniposide and paeoniflorin in Qingganning Tablet was 2-12 μg·mL^-1, 1-6 μg·mL^-1 and 4-8 μg·mL^-1, respectively, in which the linear relationships were favorable. The average sample-adding recovery rate was 100.3%, 99.6% and 99.8%, while degree of precision (RSD) was 1.0%, 0.9% and 1.3% in puerarin, geniposide and paeoniflorin, respectively. Conclusion: HPLC is simple and accurate in operation with excellent repeatability, and can detect the content of puerarin, geniposide and paeoniflorin simultaneously under the same chromatographic condition.

【 授权许可】

Unknown