A novel method for the quality control of saffron through the simultaneous analysis of authenticity and adulteration markers by liquid chromatography-(quadrupole-time of flight)-mass spectrometry

卷:228

Guijarro-Diez, Miguel ; Castro-Puyana, Maria ; Luis Crego, Antonio ; Luisa Marina, Maria

Univ Alcala De Henares

关键词: Saffron;    Quality control;    Authenticity markers;    Glycosylated kaempferols;    Geniposide;    Liquid chromatography-mass spectrometry;    QTOF;   

DOI  :  10.1016/j.foodchem.2017.02.015

学科分类：食品科学和技术

【 摘 要 】

A liquid chromatography-(quadrupole-time of flight)-mass spectrometry methodology was developed to assess the authenticity of saffron through the analysis of a group of kaempferol derivatives recently proposed as novel authenticity markers as a result of a metabolomic study of saffron (kaempferol 3-O-glucoside, kaempferol 3-O-sophoroside, kaempferol 3,7-O-diglucoside, kaempferol 3,7,4'-O-triglucoside, kaempferol 3-O-sophoroside-7-O-glucoside). Geniposide was also studied as an adulteration marker of saffron with gardenia. The optimized chromatographic conditions enabling the simultaneous separation of glycosylated kaempferols and geniposide consisted of the use of a C18 column and an elution gradient with acetonitrile and water as mobile phases (both with formic acid at 0.1%). A strategy was proposed to evaluate the minimum quantifiable adulteration percentage which was established at a 0.2% regardless of the adulterant employed. The analysis of nineteen commercial samples showed the method to be specific and suitable for saffron quality control. (C) 2017 Elsevier Ltd. All rights reserved.