Potentiation of antibiotic activity by Passiflora cincinnata Mast. front of strains Staphylococcus aureus and Escherichia coli

Saudi Journal of Biological Sciences

David C. Siebra¹  Henrique D.M. Coutinho²  Saulo R. Tintino²  Fernando G. Figueredo²  Rosimeire S. Albuquerque²  Izabel Cristina Santiago Lemos³  Irwin R.A. Menezes³  Anita O.B.P.B. Martins³  Larissa R. Oliveira³  Cicero F.B. Felipe³  Ana Luiza A. Siebra³  Marta R. Kerntopf³  Gyllyandeson A. Delmondes³  Jose Galberto M. da Costa⁴ 

[1] Federal University of Ceará, Campus Cariri, Barbalha, CE, Brazil;Laboratory of Microbiology and Molecular Biology, Regional University of Cariri, Crato, CE, Brazil;Laboratory of Pharmacology and Molecular Chemistry and Laboratory of Pharmacology of Natural Products, Center of Biological Sciences and Health, Regional University of Cariri, Crato, CE, Brazil;Laboratory of Research in Natural Products, Center of Biological Sciences and Health, University of the Region of Cariri, Crato, CE, Brazil;

关键词: Antibiotic-modifying activity;    Antimicrobial activity;    Medicinal plants;    Passiflora;    Passiflora cincinnata;    Phytotherapeutic;    Staphylococcus aureus;    Escherichia coli;   

DOI  :  10.1016/j.sjbs.2016.01.019

来源: DOAJ

【 摘 要 】

The development of new drugs from plants is an interesting alternative approach to overcoming microbial resistance. Passiflora cincinnata shows resistance to diseases and pests and a higher concentration of chemical components that may be useful in the pharmaceutical industry. We investigated the potential antimicrobial and antibiotic-modifying activity of hydroalcoholic extracts of leaves, stems, bark, pulp and seeds of P. cincinnata. The extracts were prepared by homogenization of material in 50% ethanol. Minimum inhibitory concentration (MIC) was determined by the broth dilution method, and the bacterial strains tested were Staphylococcus aureus and Escherichia coli. Antibiotic-modifying activity was evaluated against the strains S. aureus 03 and E. coli 08, using a subinhibitory concentration of extract. The antibiotics tested were: amikacin, gentamicin, ampicillin, potassium benzylpenicillin and oxacillin. The extracts did not show antimicrobial activity of clinical relevance, where the MIC was equal to or greater than 1024 μg/mL. S. aureus showed 13 events, while E. coli showed only 4 events. Among these events, 14 involved synergistic activity, potentiating the effect of the antibiotics, and only 3 events demonstrated antagonistic activity toward ampicillin. Hydroalcoholic extracts are potential antimicrobial agents when combined with conventional drugs little utilized in in vivo treatment.

【 授权许可】

Unknown