Sensors | |
DNAzyme-Functionalized R-Phycoerythrin as a Cost-Effective and Environment-Friendly Fluorescent Biosensor for Aqueous Pb2+ Detection | |
Jikui Wu1  Ningna Ren1  Ruinan Wang1  Junling Zhang2  Min Jia2  Yunfei Lu2  | |
[1] College of Food Science and Technology, Ministry of Agriculture, National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Shanghai Ocean University, Shanghai 201306, China; | |
关键词: DNAzyme; R-phycoerythrin; sensors; Pb2+; fluorescence resonance energy transfer; | |
DOI : 10.3390/s19122732 | |
来源: DOAJ |
【 摘 要 】
The sensitive detection of Pb2+ is of significant importance for food safety, environmental monitoring, and human health care. To this end, a novel fluorescent biosensor, DNAzyme-functionalized R-phycoerythrin (DNAzyme-R-PE), was presented for Pb2+ analysis. The biosensor was prepared via the immobilization of Iowa Black® FQ-modified DNAzyme−substrate complex onto the surface of SPDP-functionalized R-PE. The biosensor produced a minimal fluorescence signal in the absence of Pb2+. However, Pb2+ recognition can induce the cleavage of substrate, resulting in a fluorescence restoration of R-PE. The fluorescence changes were used to measure sensitively Pb2+ and the limit of detection was 0.16 nM with a linear range from 0.5−75 nM. Furthermore, the proposed biosensor showed excellent selectivity towards Pb2+ even in the presence of other metal ions interferences and was demonstrated to successfully determine Pb2+ in spiked lake water samples.
【 授权许可】
Unknown