Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications

Molecules

Orazio Vittorio¹  GiovanniDe Filpo²  FiorePasquale Nicoletta³  Manuela Curcio³  Giuseppe Cirillo³  Francesca Iemma³  Antonella Leggio³  Elvira Pantuso⁴ 

[1] Children’s Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Sydney, NSW 2031, Australia;Department of Chemistry and Chemical Technologies, University of Calabria, 87036 Rende (CS), Italy;Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy;National Research Council of Italy (CNR)—Institute on Membrane Technology (ITM), 87036 Rende (CS), Italy;

关键词: hybrid hydrogels;    controlled drug delivery;    protein crystallization;    lysozyme;   

DOI  :  10.3390/molecules26051355

来源: DOAJ

【 摘 要 】

In this work, we combined electrically-conductive graphene oxide and a sodium alginate-caffeic acid conjugate, acting as a functional element, in an acrylate hydrogel network to obtain multifunctional materials designed to perform multiple tasks in biomedical research. The hybrid material was found to be well tolerated by human fibroblast lung cells (MRC-5) (viability higher than 94%) and able to modify its swelling properties upon application of an external electric field. Release experiments performed using lysozyme as the model drug, showed a pH and electro-responsive behavior, with higher release amounts and rated in physiological vs. acidic pH. Finally, the retainment of the antioxidant properties of caffeic acid upon conjugation and polymerization processes (Trolox equivalent antioxidant capacity values of 1.77 and 1.48, respectively) was used to quench the effect of hydrogen peroxide in a hydrogel-assisted lysozyme crystallization procedure.

【 授权许可】

Unknown