Design and Synthesis of Chitosan—Gelatin Hybrid Hydrogels for 3D Printable in vitro Models

Frontiers in Chemistry

Giulia Beatrice Rossi¹  Francesco Nicotra¹  Laura Russo¹  Sofia Magli¹  Elisa Masseroni²  Laura Piazza²  Elisa Ballarini³  Luca Crippa³  Guido Cavaletti³  Cesare Cosentino⁴  Giulia Risi⁴  Sabrina Bertini⁴ 

[1] Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy;Department of Environmental Science and Policy (ESP), University of Milan, Milan, Italy;Department of Medical and Surgical Science, University of Milano-Bicocca, Milan, Italy;G. Ronzoni Institute for Chemical and Biochemical Research, Milan, Italy;

关键词: glycopolymers;    hybrid hydrogels;    functionalization strategies;    Diels-Alder click reaction;    3D bioprinting;    3D cultures;   

DOI  :  10.3389/fchem.2020.00524

来源: DOAJ

【 摘 要 】

The development of 3D printable hydrogels based on the crosslinking between chitosan and gelatin is proposed. Chitosan and gelatin were both functionalized with methyl furan groups. Chemical modification was performed by reductive amination with methyl furfural involving the lysine residues of gelatin and the amino groups of chitosan to generate hydrogels with tailored properties. The methyl furan residues present in both polymers were exploited for efficient crosslinking via Diels-Alder ligation with PEG-Star-maleimide under cell-compatible conditions. The obtained chitosan-gelatin hybrid was employed to formulate hydrogels and 3D printable biopolymers and its processability and biocompatibility were preliminarily investigated.

【 授权许可】

Unknown