Detection of Aβ(1-40) Protein in Human Serum as a Causative Agent of Alzheimer’s Disease by Strain Gauge Cantilever Biosensor Immobilizing Liposome Incorporating Cholesterol

Proceedings

Kaoru Yamashita¹  Minoru Noda¹  Yuki Murakami¹  Tomoya Taniguchi¹  Masayuki Sohgawa² 

[1] Kyoto Institute of Technology, Kyoto, Japan;Niigata University, Niigata, Japan;

关键词: cantilever;    NiCr strain gauge;    biosensor;    liposome;    amyloid beta;    aggregation;    fibrillization;    interaction;    human serum;    digital low-pass filtering procedure;   

DOI  :  10.3390/proceedings1040503

来源: DOAJ

【 摘 要 】

We have successfully measured amyloid beta (Aβ) (1-40) protein added in human serum by a NiCr strain gauge cantilever biosensor immobilized with liposomes incorporating cholesterol. Importantly, we investigated the effect of incorporation of cholesterol in the liposome in order to suppress the interaction between the liposome and many different proteins included in human serum. It was revealed that incorporating cholesterol suppresses the interaction between the proteins other than Aβ in human serum and the liposome. Finally, we detected Aβ(1-40) in human serum with typical chronological behaviors due to Aβ aggregation and fibrillization. Furthermore, as a digital low-pass filtering procedure could reduce external noises, the cantilever sensor immobilized with liposome incorporating cholesterol can detect low-concentrated Aβ in human serum.

【 授权许可】

Unknown