Cryo-EM analysis of Ebola virus nucleocapsid-like assembly

STAR Protocols

Grigore D. Pintilie¹  Gaya K. Amarasinghe²  Zhaoming Su²  Daisy W. Leung³  Wah Chiu³  Jennifer M. Binning⁴  Yan Wang⁵ 

[1] Department of Molecular Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA;Division of Cryo-EM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA;Department of Bioengineering and Department of Microbiology and Immunology, James H. Clark Center, Stanford University, Stanford, CA 94305, USA;Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA;The State Key Laboratory of Biotherapy and Cancer Center, Department of Geriatrics and National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610044, China;

关键词: Cryo-EM;    Microbiology;    Protein Biochemistry;    Protein expression and purification;    Structural Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: This protocol describes the reconstitution of the filamentous Ebola virus nucleocapsid-like assembly in vitro. This is followed by solving the cryo-EM structure using helical reconstruction, and flexible fitting of the existing model into the 5.8 Å cryo-EM map. The protocol can be applied to other filamentous viral protein assemblies, particularly those with high flexibility and moderate resolution maps, which present technical challenges to model building.For complete details on the use and execution of this profile, please refer to Su et al. (2018).

【 授权许可】

Unknown