Purification and cryo-EM structure determination of Arabidopsis thaliana GLR3.4

STAR Protocols

Maria V. Yelshanskaya¹  Alexander I. Sobolevsky²  Shanti Pal Gangwar²  Marriah N. Green² 

[1] Institute of Human Nutrition, Columbia University, 630 West 168th Street, New York, NY 10032, USA;Department of Biochemistry and Molecular Biophysics, Columbia University, 650 West 168th Street, New York, NY 10032, USA;

关键词: Model Organisms;    Plant sciences;    Protein Biochemistry;    Protein expression and purification;    Cryo-EM;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Ionotropic glutamate receptors (iGluRs) are ligand-gated ion channels that play crucial roles in the central nervous system. iGluR homologs, termed glutamate receptor-like channels (GLRs), have been found in plants. Investigating the structural and functional relationship between iGluRs and GLRs was limited by GLR protein expression, purification, and structural characterization. Here, we provide a detailed protocol for Arabidopsis thaliana GLR3.4 (AtGLR3.4) expression in a mammalian cell line and purification for structure determination by cryogenic electron microscopy (cryo-EM).For the complete details on the use and execution of this protocol, please refer to Green et al. (2021).

【 授权许可】

Unknown