Protocol for high-throughput cloning, expression, purification, and evaluation of bispecific antibodies

STAR Protocols

Timothy P. Riley¹  Irwin Chen²  Danqing Li³  Alexander C. Partin³  Mark L. Michaels³  Zhulun Wang³  Fernando Garces³  Danyang Gong³  Liangjun Zhao³ 

[1] Corresponding author;Department of Therapeutics Discovery, Amgen Research, Amgen Inc., San Francisco, CA 94080, USA;Department of Therapeutics Discovery, Amgen Research, Amgen Inc., Thousand Oaks, CA 91320, USA;

关键词: High Throughput Screening;    Antibody;    Protein Biochemistry;    Protein expression and purification;    Biotechnology and bioengineering;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Bispecific antibodies are a powerful new class of therapeutics, but their development often requires enormous amounts of time and resources. Here, we describe a high-throughput protocol for cloning, expressing, purifying, and evaluating bispecific antibodies. This protocol enables the rapid screening of large panels of bispecific molecules to identify top candidates for further development.For complete details on the use and execution of this protocol, please refer to Estes et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

【 授权许可】

Unknown