Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals

Microorganisms

Salma Younes¹  Nadin Younes¹  DuaaW. Al-Sadeq¹  HadiM. Yassine¹  Hadeel Al-Jighefee¹  Farah Shurrab¹  GheyathK. Nasrallah¹  Nader Al-Dewik²  Hamda Qotba³  Mohamed Syed³  Ahmed Alnuaimi³  Patrick Tang⁴  SohaR. Dargham⁵  LaithJ. Abu-Raddad⁵ 

[1] Biomedical Research Center, Qatar University, Doha P.O. Box 2713, Qatar;Clinical and Metabolic Genetics Section, Pediatrics Department, Hamad General Hospital (HGH), Hamad Medical Corporation, Doha P.O. Box 3050, Qatar;Department of Clinical Research, Primary Health Care Centers, Doha P.O. Box 26555, Qatar;Department of Pathology, Sidra Medicine, Doha P.O. Box 26999, Qatar;Infectious Disease Epidemiology Group, Weill Cornell Medicine-Qatar, Cornell University, Qatar Foundation–Education City, Doha P.O. Box 24144, Qatar;

关键词: COVID-19;    SARS-CoV-2;    serology;    sensitivity;    specificity;    neutralizing antibodies;   

DOI  :  10.3390/microorganisms9020245

来源: DOAJ

【 摘 要 】

To support the deployment of serology assays for population screening during the COVID-19 pandemic, we compared the performance of three fully automated SARS-CoV-2 IgG assays: Mindray CL-900i^® (target: spike [S] and nucleocapsid [N]), BioMérieux VIDAS^®3 (target: receptor-binding domain [RBD]) and Diasorin LIAISON^®XL (target: S1 and S2 subunits). A total of 111 SARS-CoV-2 RT-PCR- positive samples collected at ≥ 21 days post symptom onset, and 127 pre-pandemic control samples were included. Diagnostic performance was assessed in correlation to RT-PCR and a surrogate virus-neutralizing test (sVNT). Moreover, cross-reactivity with other viral antibodies was investigated. Compared to RT-PCR, LIAISON^®XL showed the highest overall specificity (100%), followed by VIDAS^®3 (98.4%) and CL-900i^® (95.3%). The highest sensitivity was demonstrated by CL-900i^® (90.1%), followed by VIDAS^®3 (88.3%) and LIAISON^®XL (85.6%). The sensitivity of all assays was higher in symptomatic patients (91.1–98.2%) compared to asymptomatic patients (78.4–80.4%). In correlation to sVNT, all assays showed excellent sensitivities (92.2–96.1%). In addition, VIDAS^®3 demonstrated the best correlation (r = 0.75) with the sVNT. The present study provides insights on the performance of three fully automated assays, which could help diagnostic laboratories in the choice of a particular assay according to the intended use.

【 授权许可】

Unknown