Characterization of the various forms of the Reelin protein in the cerebrospinal fluid of normal subjects and in neurological diseases

Neurobiology of Disease

Christian J.M Sindic¹  Nina Ignatova²  André M Goffinet² 

[1]Neurochemistry Unit, University of Louvain Medical School, B1200 Brussels, Belgium

[2]Developmental Genetics Unit, University of Louvain Medical School, B1200 Brussels, Belgium

关键词: Reelin;    Dab1;    Brain development;    Alzheimer's disease;    Autism;    Schizophrenia;   

DOI  :  

来源: DOAJ

【 摘 要 】

Reelin is a large extracellular glycoprotein that is defective in reeler mutant mice and plays a well-established role during brain development in human as well as rodents. In the adult brain, Reelin is expressed in a subset of GABAergic interneurons. Its role in disease states is not clearly defined, although it is implicated in autism and psychoses such as schizophrenia. In this report, we show that Reelin immunoreactive proteins can be detected in the human cerebrospinal fluid (CSF) with monoclonal antibodies directed against the N- and C-terminal regions of the protein. In CSF, Reelin is present as different products due to processing at two main sites; preservation at −20°C increases processing further. CSF Reelin originates from the brain tissue and not from plasma. The protein was detected in comparable concentrations in children and adults, and the signal varied largely from subject to subject with no obvious correlation with age or neurological disease state.

【 授权许可】

Unknown