Study on the Detection of the Content of the Phenylalanine by Rare Earth Ions Fluorescence Probe Method

4th Annual International Workshop on Materials Science and Engineering

Wang, Bo^1 ; Lv, Yuguang^1 ; Song, Shanshan^1 ; Zhao, Yaxin^1 ; Zhou, Ziran^1 ; Lin, Peng^1

College of Pharmacy, Jiamusi University, Jiamusi, China^1

关键词: Brain development;    Experimental conditions;    Fluorescence intensities;    Fluorescence probes;    Mental retardation;    Metabolic disease;    Optimum concentration;    Theoretical approach;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/381/1/012172/pdf
DOI  :  10.1088/1757-899X/381/1/012172

来源: IOP

【 摘 要 】

Phenylketonuria(PKU) is a kind of genetic and metabolic disease which caused by mutations. High concent of phenylalanine and its metabolites in the blood which result in impairment of brain development, even mental retardation. In recent years, due to the impact factors of diet and environment, PKU disease was significantly increased. Studies have shown that early detection of abnormal levels of phenylalanine and treatment will greatly reduce the rate of mental retardation. Therefore, monitoring of blood levels of phenylalanine is particularly important for phenylketonuria patients. This study discussed a new method of terbium rare earth ions fluorescence probe to detect the content of phenylalanine. At the same time, the experimental conditions were optimized. The results showed that the optimal pH of the system was 8, the optimum surfactant was SDBS(1.0×10-3 mol/L) and the optimum concentration of Tb3+ was 3 × 10-2 mol/L. Under optimal test conditions, the fluorescence intensity had a good liner relationship with the concentration of phenylalanine in the range of 6×10-7∼3 ×10-5 mol/L. And the linear equation as follows: F = 1.0979CPhe +23.50268, r = 0.998. It provided a new theoretical approach for the clinical test of Phenylketonuria.