Modeling human retinoblastoma using embryonic stem cell-derived retinal organoids

STAR Protocols

Zi-Bing Jin¹  Zi-Qi Hua²  Hui Liu³ 

[1] Institute of Biomedical Big Data, School of Biomedical Engineering, School of Ophthalmology &Retinal Regeneration, The Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China;;Laboratory of Stem Cell &

关键词: Cell culture;    Cancer;    Stem Cells;    Cell Differentiation;    Organoids;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Retinoblastoma (Rb) is the most prevalent intraocular malignancy in early childhood. Traditional models are unable to accurately recapitulate the origin and development of human Rb. Here, we present a protocol to establish a novel human Rb organoid (hRBO) model derived from genetically engineered human embryonic stem cells (hESCs). This hRBO model exhibits properties highly consistent with human primary Rb and can be used effectively for dissecting the origination and pathogenesis of Rb as well as for screening of potential therapies.For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).

【 授权许可】

Unknown