A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes

STAR Protocols

Phoebe Dillon¹  Kunhua Song²  Yingqiong Cao³  Walter E. Knight⁴ 

[1] Consortium for Fibrosis Research and Translation, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA;Corresponding author;Gates Center for Regenerative Medicine and Stem Cell Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA;Division of Cardiology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA;

关键词: Cell Biology;    Cell culture;    Stem Cells;    Cell Differentiation;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: When cultured under typical conditions, human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are structurally and functionally immature. We have previously demonstrated that culture of hiPSC-CMs in maturation medium containing fatty acids, in combination with culture on micropatterned surfaces, produces cells that demonstrate a more mature phenotype compared to standard approaches. Here, we show in detail the steps needed to produce mature hiPSC-CMs. Compared with many approaches, our protocol is relatively simple and can be easily adapted to new laboratories.For complete details on the use and execution of this protocol, please refer to Knight et al. (2021).

【 授权许可】

Unknown