Earlier In Vitro Viral Production With SARS-CoV-2 Alpha Than With Beta, Gamma, B, or A.27 Variants

Frontiers in Cellular and Infection Microbiology

Valentine Marie Ferré¹  Diane Descamps¹  Lucile Larrouy¹  Charlotte Charpentier¹  Nabil Benmalek¹  Gilles Collin¹  Nadhira Houhou-Fidouh¹  Benoit Visseaux¹  Yazdan Yazdanpanah²  Jean-François Timsit³  Romain Coppée⁴  Houssem Redha Chenane⁴  Samuel Lebourgeois⁴  Reyene Menidjel⁴ 

[1] Assistance Publique - Hôpitaux de Paris (AP-HP), University Hospital Bichat-Claude Bernard, Laboratoire de Virologie, Paris, France;Assistance Publique - Hôpitaux de Paris (AP-HP), University Hospital Bichat-Claude Bernard, Maladies Infectieuses et Tropicales, Paris, France;Assistance Publique - Hôpitaux de Paris (AP-HP), University Hospital Bichat-Claude Bernard, Réanimation Médicale et Infectieuses, Paris, France;Université de Paris, Infection Antimicrobials Modelling Evolution (IAME), Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France;

关键词: alpha;    SARS-CoV-2;    variants;    replication cycle;    infectious titres;   

DOI  :  10.3389/fcimb.2021.792202

来源: DOAJ

【 摘 要 】

Since its emergence in China at the end of 2019, SARS-CoV-2 has rapidly spread across the world to become a global public health emergency. Since then, the pandemic has evolved with the large worldwide emergence of new variants, such as the Alpha (B.1.1.7 variant), Beta (B.1.351 variant), and Gamma (P.1 variant), and some other under investigation such as the A.27 in France. Many studies are focusing on antibody neutralisation changes according to the spike mutations, but to date, little is known regarding their respective replication capacities. In this work, we demonstrate that the Alpha variant provides an earlier replication in vitro, on Vero E6 and A549 cells, than Beta, Gamma, A.27, and historical lineages. This earlier replication was associated with higher infectious titres in cell-culture supernatants, in line with the higher viral loads observed among Alpha-infected patients. Interestingly, Beta and Gamma variants presented similar kinetic and viral load than the other non-Alpha-tested variants.

【 授权许可】

Unknown