The Identification and Genetic Characterization of Parechovirus Infection Among Pediatric Patients With Wide Clinical Spectrum in Chongqing, China

Frontiers in Microbiology

Wei Liu¹  Xiang Tang²  Luo Ren²  Rui-Qiu Zhao²  Hong-Mei Xu²  En-Mei Liu²  Hai-Sheng Zhou³  Qin-Bin Lu⁴  Yu-Na Wang⁶  Li-Qun Fang⁶  Jin-Jin Chen⁶  Xiao-Ai Zhang⁶  Pan-He Zhang⁶  Zi-Wei Zhou⁶  Hai-Yang Zhang⁶  Yang Yuan⁶ 

[1] Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Diseases, Beijing, China;Children’s Hospital of Chongqing Medical University, Chongqing, China;Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei, China;Department of Laboratorial Science and Technology, School of Public Health, Peking University, Beijing, China;Key Laboratory of Dermatology, Anhui Medical University, Hefei, China;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China;

关键词: parechovirus;    epidemiology;    respiratory infection;    acute diarrhea;    hand foot and mouth disease;   

DOI  :  10.3389/fmicb.2021.709849

来源: DOAJ

【 摘 要 】

Human parechoviruses (HPeVs) are important causes of infection in children. However, without a comprehensive and persistent surveillance, the epidemiology and clinical features of HPeV infection remain ambiguous. We performed a hospital-based surveillance study among three groups of pediatric patients with acute respiratory infection (Group 1), acute diarrhea (Group 2), and hand, foot and mouth disease (Group 3) in Chongqing, China, from 2009 to 2015. Among 10,212 tested patients, 707 (6.92%) were positive for HPeV, with the positive rates differing significantly among three groups (Group 1, 3.43%; Group 2, 14.94%; Group 3, 3.55%; P < 0.001). The co-infection with other pathogens was detected in 75.2% (531/707) of HPeV-positive patients. Significant negative interaction between HPeV and Parainfluenza virus (PIV) (P = 0.046, OR = 0.59, 95% CI = 0.34–0.98) and positive interactions between HPeV and Enterovirus (EV) (P = 0.015, OR = 2.28, 95% CI = 1.23–4.73) were identified. Among 707 HPeV-positive patients, 592 (83.73%) were successfully sequenced, and 10 genotypes were identified, with HPeV1 (n = 396), HPeV4 (n = 86), and HPeV3 (n = 46) as the most frequently seen. The proportion of genotypes differed among three groups (P < 0.001), with HPeV1 and HPeV4 overrepresented in Group 2 and HPeV6 overrepresented in Group 3. The spatial patterns of HPeV genotypes disclosed more close clustering of the currently sequenced strains than those from other countries/regions, although they were indeed mixed. Three main genotypes (HPeV1, HPeV3, and HPeV4) had shown distinct seasonal peaks, highlighting a bi-annual cycle of all HpeV and two genotypes (HPeV 1 and HPeV 4) with peaks in odd-numbered years and with peaks in even-numbered years HPeV3. Significantly higher HPeV1 viral loads were associated with severe diarrhea in Group 2 (P = 0.044), while associated with HPeV single infection than HPeV-EV coinfection among HFMD patients (P = 0.001). It’s concluded that HPeV infection was correlated with wide clinical spectrum in pediatric patients with a high variety of genotypes determined. Still no clinical significance can be confirmed, which warranted more molecular surveillance in the future.

【 授权许可】

Unknown