An in situ-Synthesized Gene Chip for the Detection of Food-Borne Pathogens on Fresh-Cut Cantaloupe and Lettuce

Frontiers in Microbiology

Ya Long¹  Ying Lao¹  Yuanzheng Li¹  Ke Feng¹  Wenzhong Hu²  Sarengaowa²  Aili Jiang²  Sarengaowa³  Zhilong Xiu³ 

[1] College of Life Science, Dalian Minzu University, Dalian, China;Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian, China;School of Bioengineering, Dalian University of Technology, Dalian, China;

关键词: Salmonella Typhimurium;    Vibrio parahemolyticus;    Staphylococcus aureus;    Listeria monocytogenes;    Escherichia coli O157:H7;    in situ-synthesized gene chip;   

DOI  :  10.3389/fmicb.2019.03089

来源: DOAJ

【 摘 要 】

Fresh foods are vulnerable to foodborne pathogens which cause foodborne illness and endanger people’s life and safety. The rapid detection of foodborne pathogens is crucial for food safety surveillance. An in situ-synthesized gene chip for the detection of foodborne pathogens on fresh-cut fruits and vegetables was developed. The target genes were identified and screened by comparing the specific sequences of Salmonella Typhimurium, Vibrio parahemolyticus, Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli O157:H7 from the National Center for Biotechnology Information database. Tiling array probes were designed to target selected genes in an optimized hybridization system. A total of 141 specific probes were selected from 3,227 hybridization probes, comprising 26 L. monocytogenes, 24 S. aureus, 25 E. coli O157:H7, 20 Salmonella Typhimurium, and 46 V. parahemolyticus probes that are unique to this study. The optimized assay had strong amplification signals and high accuracy. The detection limit for the five target pathogens on fresh-cut cantaloupe and lettuce was approximately 3 log cfu/g without culturing and with a detection time of 24 h. The detection technology established in this study can rapidly detect and monitor the foodborne pathogens on fresh-cut fruits and vegetables throughout the logistical distribution chain, i.e., processing, cleaning, fresh-cutting, packaging, storage, transport, and sale, and represents a valuable technology that support the safety of fresh agricultural products.

【 授权许可】

Unknown