Tnf-a production and apoptosis in hepatocytes after listeria monocytogenes and salmonella typhimurium invasion

Revista do Instituto de Medicina Tropical de São Paulo

Sânia Alves Dos Santos²  Dahir Ramos De Andrade Júnior²  Dahir Ramos De Andrade¹ 

[1] ,University of Sao Paulo School of Medicine Department of Infectious DiseasesSao Paulo SP ,Brazil

关键词: Listeria monocytogenes;    Salmonella Typhimurium;    Hepatocytes;    Apoptosis;    Tumor necrosis factor-alpha;   

DOI  :  10.1590/S0036-46652011000200009

来源: SciELO

【 摘 要 】

Invasion of hepatocytes by Listeria monocytogenes (LM) and Salmonella Typhimurium (ST) can stimulate tumor necrosis factor alpha (TNF-α) release and induce apoptosis. In this study, we compared the behavior of hepatocytes invaded by three L. monocytogenes serotypes (LM-4a, LM-4b and LM-1/2a) and by ST to understand which bacterium is more effective in the infectious process. We quantified TNF-α release by ELISA, apoptosis rates by annexin V (early apoptosis) and TUNEL (late apoptosis) techniques. The cell morphology was studied too. TNF-α release rate was highest in ST-invaded hepatocytes. ST and LM-1/2a induced the highest apoptosis production rates evaluated by TUNEL. LM-4b produced the highest apoptosis rate measured by annexin. Invaded hepatocytes presented various morphological alterations. Overall, LM-4b and LM-1/2a proved to be the most efficient at cell invasion, although ST adapted faster to the environment and induced earlier hepatocyte TNF-α release.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License