Saudi Journal of Biological Sciences | |
Comparative genome sequence and phylogenetic analysis of chloroplast for evolutionary relationship among Pinus species | |
Shariat Ullah1  AzizUllah AzizUllah1  Xiukang Wang2  Sajid Fiaz3  Umar Zeb3  Khurram Shahzad4  Hanif Khan5  Habib Ali6  | |
[1] Corresponding authors.;College of Life Sciences, Yan’an University, Yan’an 716000, Shaanxi, China;Department of Biology, The University of Haripur, 22620, Pakistan;Department of Botany University of Malakand, Pakistan;Department of Plant Breeding anf Genetics, The University of Haripur, 22620 Haripur, Pakistan;Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an 710069, China; | |
关键词: Pinus; Plastid genome; Sequence differentiation; Divergence time; Divergence hotspots; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Genus Pinus is a widely dispersed genus of conifer plants in the Northern Hemisphere. However, the inadequate accessibility of genomic knowledge limits our understanding of molecular phylogeny and evolution of Pinus species. In this study, the evolutionary features of complete plastid genome and the phylogeny of the Pinus genus were studied. A total of thirteen divergent hotspot regions (trnk-UUU, matK, trnQ-UUG, atpF, atpH, rpoC1, rpoC2, rpoB, ycf2, ycf1, trnD-GUC, trnY-GUA, and trnH-GUG) were identified that would be utilized as possible genetic markers for determination of phylogeny and population genetics analysis of Pinus species. Furthermore, seven genes (petD, psaI, psaM, matK, rps18, ycf1, and ycf2) with positive selection site in Pinus species were identified. Based on the whole genome this phylogenetic study showed that twenty-four Pinus species form a significant genealogical clade. Divergence time showed that the Pinus species originated about 100 million years ago (MYA) (95% HPD, 101.76.35–109.79 MYA), in lateral stages of Cretaceous. Moreover, two of the subgenera are consequently originated in 85.05 MYA (95% HPD, 81.04–88.02 MYA). This study provides a phylogenetic relationship and a chronological framework for the future study of the molecular evolution of the Pinus species.
【 授权许可】
Unknown