An hsp70 homolog is encoded on the plastid genome of the red alga, Porphyra umbilicalis

FEBS Letters

Reith, Michael¹  Munholland, Janet¹ 

[1] Institute for Marine Biosciences, National Research Council of Canada, 1411 Oxford St., Halifax, Nova Scotia B3H 3Z1, Canada

关键词: dnaK;    Heat shock;    hsp70;    Plastid genome;    Red alga;    trnG(GCC);   

DOI  :  10.1016/0014-5793(91)81355-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A PCR experiment using Porphyra umbilicalis DNA as the template and degenerate oligonucleotides representing conserved regions of hsp70 amino acid sequences generated a 1 kb product that hybridized exclusively to the plastid DNA of this red alga. DNA sequencing of two continguous EcoRI plastid DNA clones revealed a 620 amino acid open reading frame with 71% identity to the dnaK gene of the cyanobacterium. Synechocystis 6803. Northern hybridization experiments detected a 2.3 kb transcript that is present in control (15°C) cultures and increases approximately 7-fold upon heat shock (75 minutes at 30°C).

【 授权许可】

Unknown