The expression pattern of VISTA in the PBMCs of relapsing-remitting multiple sclerosis patients: A single-cell RNA sequencing-based study

Biomedicine & Pharmacotherapy

Shima Rahmani¹  Zahra Asadzadeh²  Patrizia Leone³  Saba Pouya⁴  Mahdi Abdoli Shadbad⁵  Mohammad Ghasemigol⁶  Vito Racanelli⁷  Maryam Shojaee⁸  Afshin Derakhshani⁹  Adib Miraki Feriz⁹  Negar Hosseinkhani⁹  Haniyeh Ahmadi⁹  Elham Safarzadeh⁹  Behzad Baradaran⁹  Hossein Safarpour⁹  Neda Jalili Tabrizi^1,10  Hormoz Ayromlou^1,11 

[1] Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;Laboratory of Experimental Pharmacology, IRCCS Istituto Tumori Giovanni Paolo II, Bari, Italy;Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran;Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran;Department of Biomedical Sciences and Human Oncology, University of Bari ''Aldo Moro,'' Bari, Italy;Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran;Department of Computer Engineering, Faculty of Engineering, University of Birjand, Birjand, Iran;Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran;

关键词: Multiple sclerosis;    Immune checkpoint;    Single-cell transcriptomics;    VISTA;    Vsir;   

DOI  :  

来源: DOAJ

【 摘 要 】

Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS). Dysregulated immune responses have been implicated in MS development. Growing evidence has indicated that inhibitory immune checkpoint molecules can substantially regulate immune responses and maintain immune tolerance. V-domain Ig suppressor of T cell activation (VISTA) is a novel inhibitory immune checkpoint molecule that can suppress immune responses; however, its expression pattern in the peripheral blood mononuclear cells (PBMCs) of relapsing-remitting multiple sclerosis (RRMS) has not thoroughly been studied. Herein, we evaluated Vsir expression in PBMCs of RRMS patients and characterized the expression pattern of the Vsir in the PBMCs of MS patients. Besides, we investigated the effect of fingolimod, IFNβ-1α, glatiramer acetate (GA), and dimethyl fumarate (DMF) on Vsir expression in PBMCs of RRMS patients. Our results have shown that Vsir expression is significantly downregulated in the PBMCs of patients with RRMS. Besides, the single-cell RNA sequencing results have demonstrated that Vsir expression is downregulated in classical monocyte, intermediate monocytes, non-classical monocytes, myeloid DCs (mDC), Plasmacytoid dendritic cells (pDCs), and naive B-cells of PBMCs of MS patients compared to the control. In addition, DMF, IFNβ-1α, and GA have significantly upregulated Vsir expression in the PBMCs of RRMS patients. Collectively, the current study has shed light on Vsir expression in the PBMCs of MS patients; however, further studies are needed to elucidate the significance of VISTA in the mentioned immune cells.

【 授权许可】

Unknown