Transient Astrocytic Gq Signaling Underlies Remote Memory Enhancement

Frontiers in Neural Circuits

Tsuneko Mishima¹  Sonam Akther¹  Hajime Hirase¹  Ashley Bomin Lee¹  Camilla Trang Vo²  Shigeyoshi Itohara³  Mika Tanaka³  Youichi Iwai⁴  Kazuko Yahagi⁴  Katsuya Ozawa⁴ 

[1] Center for Translational Neuromedicine, Faculty of Medical and Health Sciences, University of Copenhagen, Copenhagen, Denmark;Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark;Laboratory for Behavioral Genetics, RIKEN Center for Brain Science, Wako, Japan;Laboratory for Neuron-Glia Circuitry, RIKEN Center for Brain Science, Wako, Japan;

关键词: astrocytes;    optogenetic activation;    G protein-coupled receptor;    Ca2+ signaling;    memory;    OptoA1AR;   

DOI  :  10.3389/fncir.2021.658343

来源: DOAJ

【 摘 要 】

Astrocytes elicit transient Ca2+ elevations induced by G protein-coupled receptors (GPCRs), yet their role in vivo remains unknown. To address this, transgenic mice with astrocytic expression of the optogenetic Gq-type GPCR, Optoα1AR, were established, in which transient Ca2+ elevations similar to those in wild type mice were induced by brief blue light illumination. Activation of cortical astrocytes resulted in an adenosine A1 receptor-dependent inhibition of neuronal activity. Moreover, sensory stimulation with astrocytic activation induced long-term depression of sensory evoked response. At the behavioral level, repeated astrocytic activation in the anterior cortex gradually affected novel open field exploratory behavior, and remote memory was enhanced in a novel object recognition task. These effects were blocked by A1 receptor antagonism. Together, we demonstrate that GPCR-triggered Ca2+ elevation in cortical astrocytes has causal impacts on neuronal activity and behavior.

【 授权许可】

Unknown