Adherence of human neutrophils changes Ca2+ signaling during activation with opsonized particles

FEBS Letters

Hansen, Carl A.¹  HooyBrink, Berend²  Koenderman, Leo²  Roos, Dirk²  Tool, Anton T.J.²  Williamson, John R.¹  Verhoeven, Arthur J.² 

[1] Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA;Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, Amsterdam, The Netherlands

关键词: Neutrophil;    Adherence;    Ca2+ signaling;    STZ;    serum-treated zymosan;    [Ca2+]i;    cytosolic free Ca2+ concentration;    fMLP;    formyl-methionyl-leucyl-phenylalanine;    PIPz;    phosphatidylinositol (4;    5)-bisphosphate;    PK-C;    protein kinase C;   

DOI  :  10.1016/0014-5793(90)81232-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Changes in the cytosolic free Ca²⁺ concentration ([Ca²⁺]_i) upon activation of human neutrophils by opsonized particles (serum-treated zymosan; STZ) were evaluated by three different methods: (i) measurement of total fluorescence changes in indo-1 loaded neutrophils activated in suspension; (ii) measurement of fluorescence changes in individual indo-1 loaded neutrophils in a flow cytometer and (iii) measurement of fluorescence changes in individual fura-2 loaded neutrophils adherent to serum-coated coverslips. Our study shows that the opsonized particle-induced change in [Ca²⁺]_i in neutrophils is altered during adherence of the cells to a serum-coated surface. These observations might be of importance for neutrophil function in vivo, since adherence is a prerequisite for diapedesis and chemotaxis.

【 授权许可】

Unknown