Purification and MIC analysis of antimicrobial proteins from Cucumis sativus L. seeds

BMC Complementary and Alternative Medicine

Ayesha Mateen¹  Rabbani Syed¹  Raid Al Akeel¹  Khalid K. Alharbi¹  Abdullah A. Alyousef¹  Hazem M. Al-Mandeel² 

[1] Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University;Department of Obstetrics, Gynecology, King Khalid University Hospital & College of Medicine, King Saud University;

关键词: Antimicrobial proteins;    Cucumis sativus L;    Ion exchange chromatography;   

DOI  :  10.1186/s12906-018-2176-y

来源: DOAJ

【 摘 要 】

Abstract Background Cucumis sativus L. (cucumber), from the family Cucurbitaceae, is a therapeutic plant with various pharmacological benefits, broadly utilized as a part of complementary medicine (e.g., Unani, Ayurveda, Siddha, and Traditional Chinese). In light of past research discoveries, this plant had been chosen to consider its potential antibacterial action. Methods Extracts were purified by dialysis and ion exchange chromatography strategy and then assayed for antibacterial activity against four standard pathogenic bacterial strains known to cause foodborne infections and spoilage of food and herbal drugs. Antimicrobial peptides were extracted from seeds using a sodium phosphate citrate (pH 7.2) - CTAB cradle (pH 6.0). Results The highest protein concentration was seen with elute fractions 1 and 3 (370 mg/mL) compared with elute fractions 2 and 4 (340 mg/mL). Among the bacteria utilized, E. coli was clearly the most sensitive out of selected four strains. Conclusion Our results suggest that Cucumis sativus L seeds extracts have significant potentials as new antimicrobial agents.

【 授权许可】

Unknown