Identification of a novel antibacterial protein from hemolymph of freshwater zooplankton Mesocyclops leuckarti

Saudi Journal of Biological Sciences

Naiyf S. Alharbi¹  Sournamanickam Venkatalakshmi²  Varadhan Praveena²  Shine Kadaikunnan³  Marimuthu Govindarajan³  Jamal M. Khaled³ 

[1] Corresponding authors at: Department of Zoology, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India (M. Govindarajan).;Centre for Animal Studies, Department of Zoology, Government College for Women (Autonomous), Kumbakonam 612 001, Tamil Nadu, India;Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;

关键词: Mesocyclops leuckarti;    Zooplankton;    Hemolymph;    Antimicrobial proteins;    SDS-PAGE;   

DOI  :  

来源: DOAJ

【 摘 要 】

Bacterial infections are the most important problem of health care worldwide. The hemolymph antibacterial proteins of Mesocyclops leuckarti was isolated for the first time and its antibacterial efficacy was evaluated against four different human pathogenic microbes viz., Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia and Shigella flexneri. The antibacterial potential of the antimicrobial proteins of hemolymph samples from plankton cultured in water enriched with Cow Urine Distillate (CUD) was compared with normal ones. The results indicated that the hemolymph proteins were more potential against Gram negative bacteria than Gram positive bacteria. Klebsiella pneumonia was more susceptible to the hemolymph proteins exhibiting a zone of inhibition measuring 27 mm. The supplement of CUD to the culture media further enriched the antibacterial activity of the hemolymph proteins (29 mm). The SDS-PAGE analysis indicated two different types of clear bands representing proteins of 53 kDa and 19 kDa. Overall, this investigation signified that the microcrustaceans have a defence mechanism hemolymph of Mesocyclops leuckarti have a potential agent for novel antibiotics.

【 授权许可】

Unknown