Evaluation of the Effect of Ascorbic Acid Administration on Gene Expression Level of IL-6 and TNF-α Cytokines in Deceased Donors

Iranian Journal of Allergy, Asthma and Immunology

Soheila Milani¹  Mohammad Bagher Khosravi¹  Jafar Mehrabi Sisakht²  Seyed Mohammad Bagher Tabei³  Katayoun Najafizadeh⁴  Masoumeh Kazemi⁵ 

[1] Anesthesiology and Critical Care Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.;Department of Medical Genetics, Shiraz University of Medical Sciences, Shiraz, Iran.;Department of Medical Genetics, Shiraz University of Medical Sciences, Shiraz, Iran AND Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.;Department of Pulmonary Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.;Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran AND Department of Medical Genetics, Shiraz University of Medical Sciences, Shiraz, Iran.;

关键词: Antioxidants;    Ascorbic Acid;    Brain death;    Cytokines;    Inflammation;    Liver transplantation;   

DOI  :  

来源: DOAJ

【 摘 要 】

Brain death is associated with increased inflammatory cytokines levels and poor graft quality to transplant. We aimed to evaluate the impact of Ascorbic Acid (AA) on the inflammatory status of Brain-Dead Donors (BDDs). Forty BDDs were randomly divided into two groups. Donor treatment (n=20) consisted of 100 mg/kg AA infusion 6 hours before donor operation and subsequent infusion of 100 mg/kg/p6h until organ removal. Blood samples were taken at three times, 6 hours before donor surgery (TP(1)), immediately after laparotomy (TP(2)), and before organ removal (TP(3)). Gene expression level and serum concentration of IL-6 and TNF-α cytokines were assessed by real-time PCR and ELISA methods. To investigate transplanted liver function, serum values of Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), and Billirubin-Total were evaluated on the 1(st), 3(rd), and 10(th) postoperative days. We found a significant reduction in IL-6 mRNA expression ratio of TP(3) to TP(1) following AA application among BDDs. Despite the considerable decrease in treated donors regarding IL-6 mRNA expression ratio of TP(2) to TP(1), TP(3) to TP(2), and also TNF-α variations in these periods, the results were not significant. Regarding serum concentration of these cytokines, particularly IL-6, there was a decrease between TP(2) and TP(3) following AA application in the treated donors. Furthermore, a significant reduction was found in serum AST and ALT levels in the recipients of treated group on the 3(rd) day compared to the 1(st) day after transplantation. It seems that AA beneficially affects the inflammatory status of BDDs, resulting in improved primary allograft function.

【 授权许可】

Unknown