Antioxidative Effects of Chrysoeriol via Activation of the Nrf2 Signaling Pathway and Modulation of Mitochondrial Function

Molecules

SoYeon Kwon¹  MyungHee Kim²  DaeYu Kim²  WooDuck Seo³  So-Yeun Woo³ 

[1] Department of Mechanical Engineering, College of Engineering, Inha University, Incheon 22212, Korea;Inha Research Institute for Aerospace Medicine, Inha University, Incheon 22212, Korea;Rural Development Administration, National Institute of Crop Science, Wanju-Gun, Jeollabuk-do 55365, Korea;

关键词: chrysoeriol;    antioxidants;    mitochondrial function;    retinal pigment epithelium;    age-related macular degeneration;   

DOI  :  10.3390/molecules26020313

来源: DOAJ

【 摘 要 】

Retinal pigment epithelium (RPE) cell dysfunction caused by excessive oxidative damage is partly involved in age-related macular degeneration, which is among the leading causes of visual impairment in elderly people. Here, we investigated the protective role of chrysoeriol against hydrogen peroxide (H₂O₂)-induced oxidative stress in RPE cells. The cellular viability, reactive oxygen species (ROS) generation, and mitochondrial function of retinal ARPE-19 cells were monitored under oxidative stress or pre-treatment with chrysoeriol. The expression levels of mitochondrial-related genes and associated transcription factors were assessed using reverse transcription–quantitative polymerase chain reaction (RT-qPCR). Moreover, the protein expression of antioxidant signal molecules was characterized by Western blot analysis. Chrysoeriol significantly increased cell viability, reduced ROS generation, and increased the occurrence of antioxidant molecules in H₂O₂-treated ARPE-19 cells. Additionally, mitochondrial dysfunction caused by H₂O₂-induced oxidative stress was also considerably diminished by chrysoeriol treatment, which reduced the mitochondrial membrane potential (MMP) and upregulated mitochondrial-associated genes and proteins. Chrysoeriol also markedly enhanced key transcription factors (Nrf2) and antioxidant-associated genes (particularly HO-1 and NQO-1). Therefore, our study confirms the protective effect of chrysoeriol against H₂O₂-induced oxidative stress in RPE cells, thus confirming that it may prevent mitochondrial dysfunction by upregulating antioxidant-related molecules.

【 授权许可】

Unknown