Pericellular Oxygen Monitoring during Low-Level Light Therapy in Cell Culture Using a Microsensor System

Proceedings

Peter Dungel¹  Jochen Kieninger²  Julia Marzioch²  Friederike Kramer²  Gerald A. Urban² 

[1] Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria;University of Freiburg, Freiburg, Germany;

关键词: pericellular oxygen measurement;    electrochemical;    low-level light therapy;    cell culture;   

DOI  :  10.3390/proceedings1040499

来源: DOAJ

【 摘 要 】

An electrochemical microsensor system to monitor the pericellular oxygen concentration of fibroblasts during low-level light therapy in vitro was developed. The system provides in-sight into the metabolism of the cells during and in consequence of illumination with visible red light. This approach is a unique method for real-time investigations of cellular respiration during light therapy. The presented sensor system features direct amperometric measurements by using chronoamperometric protocols for long-term stability. The oxygen measurements do not show a disturbance by light.

【 授权许可】

Unknown