Pericellular Oxygen Monitoring during Low-Level Light Therapy in Cell Culture Using a Microsensor System

Proceedings

Marzioch, Julia¹ 

关键词: pericellular oxygen measurement;    electrochemical;    low-level light therapy;    cell culture;   

DOI  :  10.3390/proceedings1040499

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

An electrochemical microsensor system to monitor the pericellular oxygen concentration of fibroblasts during low-level light therapy in vitro was developed. The system provides in-sight into the metabolism of the cells during and in consequence of illumination with visible red light. This approach is a unique method for real-time investigations of cellular respiration during light therapy. The presented sensor system features direct amperometric measurements by using chronoamperometric protocols for long-term stability. The oxygen measurements do not show a disturbance by light.

【 授权许可】

CC BY