Non-Invasive Measurement of Thyroid Hormones in Domestic Rabbits

Animals

Natalia Sowińska¹  Łukasz Migdał²  Sylwia Pałka²  Michał Kmiecik²  Maria Chmurska-Gąsowska³  Joanna Lenarczyk-Knapik⁴ 

[1] Department of Genetics and Animal Breeding, Faculty of Veterinary Medicine and Animal Science, Poznan University of Life Sciences PULS, 60-637 Poznan, Poland;Department of Genetics, Animal Breeding and Ethology, Faculty of Animal Sciences, University of Agriculture in Krakow, 30-059 Krakow, Poland;Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-UA, University of Agriculture in Krakow, 30-059 Krakow, Poland;Veterinary Clinic Multivet, Feliksa Konecznego 6/12u, 31-216 Krakow, Poland;

关键词: triiodothyronine;    thyroxine;    urine;    feces;    serum;   

DOI  :  10.3390/ani11051194

来源: DOAJ

【 摘 要 】

Thyroid hormones are essential for metabolism, energy homeostasis and reproduction. Hormones can be measured in various biological source materials: blood, feces, urine, saliva and others. The aim of our study was to verify usefulness of thyroid hormone analysis in the urine and feces of the domestic rabbit (Oryctolagus cuniculus f. domesticus), comparing them with the serum analyses. Samples were collected from 27 does in the age of 12–14 weeks. Total thyroxine (tT4), total triiodothyronine (tT3), free thyroxine (fT4) and free triiodothyronine (fT3) were tested using the radioimmunological method in serum, feces and urine. The highest concentration of tT4 was found in feces (104.72 ± 59.52 nmol/mg) and the lowest in urine (3.03 ± 3.11 nmol/mL). The highest tT3 concentration was found in blood serum (3.19 ± 0.64 nmol/L) and the lowest in urine (0.31 ± 0.43 nmol/L). The highest concentration of fT4 was observed in feces (43.71 ± 4.79 pmol/mg) and the lowest in blood serum (14.97 ± 3.42 pmol/L). The statistically highest concentration of fT3 (28.56 ± 20.79 pmol/L) was found in urine, whereas the lowest concentration of this hormone was found in feces (3.27 ± 1.33 pmol/mg). There was a positive and statistically significant correlation between serum and urine fT3 (r = 0.76) and a high positive correlation between serum and feces fT3 concentration (r = 0.62). Correlations between concentrations of other thyroid hormones between serum, urine and feces were found to be insignificant. The results suggest that fT3 can be accurately and reliably measured in the feces and urine of the domestic rabbit.

【 授权许可】

Unknown