Effect of Neonicotinoids on Bacterial Symbionts and Insecticide-Resistant Gene in Whitefly, Bemisia tabaci

Insects

Snigdha Samanta¹  Arunava Samanta¹  Mritunjoy Barman¹  Himanshu Thakur²  Jayanta Tarafdar³  Swati Chakraborty³  Amalendu Ghosh⁴ 

[1] Department of Agricultural Entomology, BCKV, Mohanpur 721436, India;Department of Entomology, C.S.K. Himachal Pradesh Krishi Vishvavidyalaya, Palampur 176062, India;Department of Plant Pathology, BCKV, Nadia, Kalyani 741245, India;Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India;

关键词: qPCR;    gene expression;    cytochrome P450;    Portiera;    Rickettsia;    management;   

DOI  :  10.3390/insects12080742

来源: DOAJ

【 摘 要 】

The silverleaf whitefly, Bemisia tabaci (Gennadius, Hemiptera: Aleyrodidae), is a major threat to field and horticultural crops worldwide. Persistent use of insecticides for the management of this pest is a lingering problem. In the present study, the status of sensitivity of B. tabaci to two neonicotinoids, imidacloprid and thiamethoxam, was evaluated. The expression pattern of two cytochrome P450 (cyp) genes and changes in the relative amount of symbionts in insecticide-treated B. tabaci were also assessed. Quantitative PCR (qPCR) studies indicate that the CYP6CM1 and CYP6CX1 genes were always expressed higher in imidacloprid-treated whitefly, suggesting a correlation between gene expression and the insect’s ability to detoxify toxic compounds such as insecticides. In addition, the thiamethoxam-treated population harbored higher Portiera and lower Rickettsia titers, whereas the imidacloprid-treated population harbored more Rickettsia at different time intervals. Interestingly, we also examined that an increase in exposure to both the insecticides resulted in a reduction in the mutualistic partners from their insect host. These differential responses of endosymbionts to insecticide exposure imply the complex interactions among the symbionts inside the host insect. The results also provide a deeper understanding of the molecular mechanism of resistance development that might be useful for formulating effective management strategies to control B. tabaci by manipulating symbionts and detoxifying genes.

【 授权许可】

Unknown