Veterinary Sciences | |
First Isolation and Multilocus Sequence Typing of Brucella canis from a Subclinically Infected Pet Dog in China | |
Guangwen Yan1  Liuhong Shen2  Zhihua Ren2  Yan Luo2  Ya Wang2  Zhijun Zhong2  Haifeng Liu2  Zidong Pang2  Guangneng Peng2  Yan Hu2  Xiaoping Ma2  Dongjie Cai2  Wei Li2  Yanqiu Zhu2  Liping Gou2  Suizhong Cao2  Yalin Zhong2  Xianpeng Shi2  Ziyao Zhou2  | |
[1] College of Animal Science, Xichang University, Xichang 615000, China;Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; | |
关键词: B. canis; pet dog; subclinical infection; public health; MLST; | |
DOI : 10.3390/vetsci9010022 | |
来源: DOAJ |
【 摘 要 】
Canine brucellosis, a worldwide zoonotic disease, is mainly caused by Brucella canis. In the present study, we isolated a Brucella strain (CD3) from a subclinically infected pet dog in Sichuan Province, Southwestern China. Classical biotyping methods and molecular biological tests (BCSP31 and BcSS PCR) proved that the strain belonged to B. canis. Furthermore, B. canis CD3 and another two B. canis strains (WJ5 and YA4), which were all isolated from pet dogs in Sichuan, were genotyped using multilocus sequence typing (MLST). Our results showed that the three B. canis strains were identified as the same sequence type (ST21). The present study is the first to report B. canis strain from a subclinically infected pet dog in China, indicating a potential threat to public health posed by subclinical infections in pet dogs. We suggest that screening for B. canis should be incorporated into routine medical examination of pet dogs and other companion animals in areas with a history of animal or human brucellosis.
【 授权许可】
Unknown