期刊论文详细信息
Frontiers in Microbiology 卷:8
First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China
Junlong Zhao1  Yuan Huang1  Jinfang Hu1  Xiaoyan Miao1  Ngabu Malobi1  Lan He1  Pei He1  Junwei He1  Long Yu1  Ligang Shi2 
[1] Key Laboratory for Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural UniversityWuhan, China;
[2] Luoyang Center for Animal Disease Control and PreventionLuoyang, China;
[3] State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, China;
关键词: Babesia gibsoni;    babesiosis;    reverse line blot;    18S rRNA;    pet dog;    companion animal;   
DOI  :  10.3389/fmicb.2017.01577
来源: DOAJ
【 摘 要 】

Canine piroplasmosis is a significant disease in dogs caused by Babesia and Theileria parasites. The clinical manifestations range from mild illness to serious disease depending on the parasite species and the physical condition of the infected dog. Canine piroplasmosis has been reported to be prevalent in China. However, no molecular evidence of the disease has been reported in pet dogs from Wuhan. In this study, 118 blood samples were randomly collected from pet dogs in veterinary clinics. The blood samples were subjected to both microscopic examination and reverse line blot (RLB) hybridization assays to detect piroplasm infection. Parasites were observed in 10 blood samples via microscopic examination, whereas there were 14 Babesia gibsoni-positive RLB tests. Phylogenetic analysis was performed after the 18S rRNA and ITS gene sequences from the 14 positive samples were cloned and sequenced. The results confirmed the existence of B. gibsoni in this area. This is the first molecular report of canine babesiosis in pet dogs from Wuhan, China. Pet dogs are companion animals, and the prevalence of babesiosis will be of concern in daily life. This study will help veterinarians better understand the prevalence of canine babesiosis and provide a guide for disease control in pet dogs.

【 授权许可】

Unknown   

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