Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis

Memórias do Instituto Oswaldo Cruz

Carlos An Ramos²  Flábio R Araújo¹  Ingrid If Souza¹  Renato Hm Oliveira¹  Carina Elisei¹  Cleber O Soares¹  Ana Ms Sacco¹  Grácia Ms Rosinha¹  Leucio C Alves¹ 

[1] ,Universidade Federal Rural de Pernambuco Departamento de Medicina Veterinária Recife PE ,Brasil

关键词: Babesia bovis;    babesiosis;    bovine;    recombinant ribosomal phosphoprotein;   

DOI  :  10.1590/S0074-02762009000700010

来源: SciELO

【 摘 要 】

Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis.

【 授权许可】

CC BY   
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