Non-Productive Infection of Glial Cells with SARS-CoV-2 in Hamster Organotypic Cerebellar Slice Cultures

Viruses

Darwyn Kobasa¹  Heidi Wood²  Babu Sajesh²  Sarah J. Medina²  Lise Lamoureux²  Jessy A. Slota²  Kathy L. Frost²  Stephanie A. Booth²  Matthew Mayor²  Kathy Manguiat²  Bryce Warner³ 

[1] Department of Medical Microbiology and Infectious Diseases, Faculty of Health Sciences, University of Manitoba, 730 William Ave., Winnipeg, MB R3E 0W3, Canada;One Health Division, Public Health Agency of Canada, National Microbiology Laboratory, 1015 Arlington St., Winnipeg, MB R3E 3R2, Canada;Special Pathogens, Public Health Agency of Canada, National Microbiology Laboratory, 1015 Arlington St., Winnipeg, MB R3E 3R2, Canada;

关键词: SARS-CoV-2;    COVID-19;    brain;    organotypic culture;    astrocytes;    microglia;   

DOI  :  10.3390/v14061218

来源: DOAJ

【 摘 要 】

The numerous neurological syndromes associated with COVID-19 implicate an effect of viral pathogenesis on neuronal function, yet reports of direct SARS-CoV-2 infection in the brain are conflicting. We used a well-established organotypic brain slice culture to determine the permissivity of hamster brain tissues to SARS-CoV-2 infection. We found levels of live virus waned after inoculation and observed no evidence of cell-to-cell spread, indicating that SARS-CoV-2 infection was non-productive. Nonetheless, we identified a small number of infected cells with glial phenotypes; however, no evidence of viral infection or replication was observed in neurons. Our data corroborate several clinical studies that have assessed patients with COVID-19 and their association with neurological involvement.

【 授权许可】

Unknown