Impact of Particle Size on Toxicity, Tissue Distribution and Excretion Kinetics of Subchronic Intratracheal Instilled Silver Nanoparticles in Mice

Toxics

Fernanda Rosário¹  Helena Oliveira¹  Conceição Santos²  Peter Hoet³  Jan Creylman⁴  Geert Verheyen⁴  Sabine Van Miert⁴ 

[1] Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal;Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal;Occupational and Environmental Toxicology, KU Leuven, ON1 Campus Gasthuisberg, Herestraat 49, 3000 Leuven, Belgium;RADIUS Group, Thomas More University College, Campus Kempen, Kleinhoefstraat 4, 2440 Geel, Belgium;

关键词: intratracheal instillation;    pulmonary exposure;    mice;    PBPK model;    silver distribution;    excretion;   

DOI  :  10.3390/toxics10050260

来源: DOAJ

【 摘 要 】

The unique physicochemical properties of silver nanoparticles (AgNPs) make them useful in a wide range of sectors, increasing their propensity for human exposure, as well as the need for thorough toxicological assessment. The biodistribution of silver, hematological parameters and GSH/GSSG levels in the lung and liver were studied in mice that were intratracheally instilled with AgNP (5 and 50 nm) and AgNO₃ once a week for 5 weeks, followed by a recovery period of up to 28 days (dpi). Data was gathered to build a PBPK model after the entry of AgNPs into the lungs. AgNPs could be absorbed into the blood and might cross the physiological barriers and be distributed extensively in mice. Similar to AgNO₃, AgNP5 induced longer-lasting toxicity toward blood cells and increased GSH levels in the lung. The exposure to AgNP50 increased the GSH from 1 dpi onward in the liver and silver was distributed to the organs after exposure, but its concentration decreased over time. In AgNP5 treated mice, silver levels were highest in the spleen, kidney, liver and blood, persisting for at least 28 days, suggesting accumulation. The major route for excretion seemed to be through the urine, despite a high concentration of AgNP5 also being found in feces. The modeled silver concentration was in line with the in vivo data for the heart and liver.

【 授权许可】

Unknown