Suppression of Airway Allergic Reactions by a Photocatalytic Filter Using Mouse Model

Toxics

Etsushi Kuroda¹  Kazuhiro Yatera²  Chinatsu Nishida²  Kaori Kato²  Hiroto Izumi³  Taisuke Tomonaga³  Yasuo Morimoto³ 

[1] Department of Immunology, Hyogo College of Medicine, 1-1 Mukogawacho, Nishinomiya 663-8131, Japan;Department of Respiratory Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu 807-8555, Japan;Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu 807-8555, Japan;

关键词: photocatalyst;    ovalbumin;    allergic reactions;    high-velocity oxygen fuel spraying;    TiO₂;    intratracheal instillation;   

DOI  :  10.3390/toxics10010040

来源: DOAJ

【 摘 要 】

Photocatalytic filters installed in air purifiers have been used to purify spaces by decomposing allergenic substances. However, we have not found any reports that evaluate the effectiveness of photocatalytic filters in suppressing allergic reactions in living organisms. In this study, we intratracheally instilled ovalbumin (OVA) into OVA-sensitized mice after the OVA was photocatalyzed by a titanium dioxide (TiO₂) filter, and verified the experimental model for evaluating the allergy-suppressing effect of photocatalysts. Mice were sensitized to OVA (10 µg/mouse) four times, and were intratracheally instilled with OVA (10 µg/mouse) after photocatalysis three times. Non-sensitized animals were instilled with normal saline following the same exposure schedule. The mice were dissected 24 h after final exposure. The OVA after photocatalysis significantly decreased the number of eosinophils in bronchoalveolar lavage fluid, and the concentration of OVA-specific IgE and IgG1 in serum, which were elevated in untreated OVA. Moreover, our experimental model showed the suppression of allergic reactions in mice, along with the decomposition of OVA after photocatalysis using the photocatalytic filter. Taken together, our experimental model for evaluating allergic reactions in the respiratory tract suggested that the allergy-suppressing effect of the photocatalytic filter can be evaluated.

【 授权许可】

Unknown