Expression on human eosinophils of CD148: a membrane tyrosine phosphatase. Implications in the effector function of eosinophils

Journal of Leukocyte Biology

Esther Aceituno²  Pilar Palomino²  Soledad Gallardo²  Ignacio Arrieta²  Fiorella Pirotto¹  Blanca Cárdaba²  Carlos Lahoz²  Isabel Cortegano²  Marta Rojo²  Antoni Gaya¹  Victoria del Pozo² 

[1] Servei d’Immunologia, Hospital Clinic, Barcelona, Spain Servei d’Immunologia, Hospital Clinic, Barcelona, Spain Servei d’Immunologia, Hospital Clinic, Barcelona, Spain;Immunology Department, Fundación Jiménez Díaz, Madrid, Spain; and Immunology Department, Fundación Jiménez Díaz, Madrid, Spain; and Immunology Department, Fundación Jiménez Díaz, Madrid, Spain; and

关键词: eosinophils;    protein kinases/phosphatases;    inflammatory mediators;    superoxide anion;    allergic reactions;   

DOI  :  

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

The role of protein tyrosine phosphatases (PTP) is crucial in regulating the phosphorylation status of cells. CD148 is a recently described membrane-type PTP. In this study, we have demonstrated that this molecule is expressed on human eosinophils and eosinophilic cell line EoL-3. Interestingly, our data also showed that this molecule acts as a transduction molecule on these cells. Thus, the crosslinking of CD148 was able to induce the degranulation and the induction of superoxide anion generation. By using specific inhibitor and by western blotting, we have shown that tyrosine kinase activation is involved in this transduction pathway. In addition, we have shown the presence of a serine/threonin kinase activity associated with CD148. In conclusion, the activation capacity of CD148 on eosinophils suggests a potential role of this molecule on inflammatory diseases, such as allergic and parasitic diseases, associated with eosinophilia.

【 授权许可】

Unknown