Mitogen-activated protein kinases p38 and ERK1/2 regulated control of Mycobacterium avium replication in primary murine macrophages is independent of tumor necrosis factor-α and interleukin-10:

Innate Immunity

KirstenKlug¹ 

关键词: macrophages;    mycobacteria;    signal transduction;    protein kinases/phosphatases;    cellular activation;    infectious immunity-bacteria;    cytokines;    mitogen-activated protein kinases;   

DOI  :  10.1177/1753425910377799

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

In macrophages, mitogen-activated protein kinases (MAPK) are critical regulators of both, mycobacterial replication and mycobacteria-induced cytokine formation. To segregate direct effects of MAPK function on mycobacterial replication from indirect, cytokine-mediated effects, we studied the growth of Mycobacterium avium strains in wild-type and tumor necrosis factor (TNF)-α- or interleukin (IL)-10-deficient bone marrow-derived murine macrophages. Using specific inhibitors of the p38- and the ERK1/2-MAPK pathways, we found that the use of SB203580 always reduced, whereas the presence of PD98059 always promoted, bacterial replication of highly virulent and intermediately virulent M. avium strains, independent of endogenous TNF-α or IL-10. The exogenous addition of TNF-α to TNF-α-deficient and wild-type M. avium-infected macrophages overrode the replication-reducing effect of SB203580, but not the replication-promoting effect of PD98059. In summary, our data demonstrate that a proper balance of MAPK activity...

【 授权许可】

CC BY